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The fischer LacZ insert was PCR increased via UAS-nucLacZ genomic jigs (Bloomington Investment Middle) employing oligos genUASFOR and genUASsv40REV. To find out which cloning web sites were utilized in your cloning involving UAS-nucLacZ, your PCR fragment ended up being sequenced employing UASFOR-SEQ and also SV40REV-SEQ. The particular KpnI/XbaI waste nucLacZ PCR fragment was then ligated straigh

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