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The nuclear LacZ put in has been PCR zoomed from UAS-nucLacZ genomic lures (Bloomington Share Centre) employing oligos genUASFOR and also genUASsv40REV. Which usually cloning websites were utilized in your cloning involving UAS-nucLacZ, the PCR fragment has been sequenced using UASFOR-SEQ and also SV40REV-SEQ. The KpnI/XbaI waste nucLacZ PCR fragment was then ligated directly into pQUAST. Mammalia

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