The fischer LacZ insert was PCR increased via UAS-nucLacZ genomic travels (Bloomington Stock Middle) using oligos genUASFOR and also genUASsv40REV. To determine which cloning websites were set up in the cloning of UAS-nucLacZ, your PCR fragment had been sequenced making use of UASFOR-SEQ and also SV40REV-SEQ. The particular KpnI/XbaI waste nucLacZ PCR fragment was then ligated in to pQUAST. Mammal


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