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, 2008; Castell�� et al., 2009). Furthermore, purified HRV 2 2A is able to cleave recombinant Nup 62 in vitro. This cleavage event liberates an FG-rich region, a domain important for nuclear transport receptor association during transport through the NPC, of Nup 62 during infection (Park et al., 2010). Interestingly, experiments utilizing recombinant 2A from the three different HRV clades
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5?mM isobutylmethylxanthine (Sigma). Following 2?days, cells were maintained within medium made up of FBS simply. For insulin legislation experiments, fully classified 3T3-L1 adipocytes have been incubated in serum-free DMEM containing 1% junk acid-free bovine solution albumin (BSA) (Sigma) along with and also with no bovine blood insulin with the dosages along with times pointed out. #links# Irf4
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